CryoLetters

ABSTRACT ARCHIVE

Abstracts: CryoLetters 35 (5), 2014

CryoLetters is a bimonthly, international journal for low temperature science and technology

 

 

Volume 35, No. 5 September/October 2014

ISSN 0143-2044

 

 


Effect of fasudil hydrochloride on the post-thaw viability of cryopreserved porcine adipose-derived stem cells
Yuntao Ji, Deyu Chen, Shuanglin Jiang and Changqing Qu

356-360

 

 


Efficacy of fish embryo vitrification protocols in terms of embryo morphology — a systematic review
Aline Ferreira Souza de Carvalho,
Shayenne Elizianne Ramos,
Thatijanne Santos Gonzaga de Carvalho,
Yasmin Chalfoun Pomárico de Souza,
Márcio Gilberto Zangeronimo, Luciano José Pereira and
Luis David Solis Murgas

361-370

 

 


Effect of ultrasonic waves on the freezing rates of potatoes in degassed coolant and untreated coolant
Deyang Yu and Baolin Liu

371-376

 

 


Development of an encapsulation-vitrification protocol for Rubia akane (Nakai) hairy roots: a comparison with non-encapsulation
Dong-Jin Shin, Hyo-Eun Lee, Chang-Hwan Bae,
Sang-Un Park, Ho-Nam Kang and Haeng-Hoon Kim

377-384

 

 


Cryopreservation of dormant in vivo-buds of hybrid aspen: timing as critical factor
Tuija Aronen and Leena Ryynänen

385-394

 

 


Extracellular ice phase transitions in insects
Timothy C. Hawes

395-399

 

 


Production of organ extracellular matrix using a freeze-thaw cycle employing extracellular cryoprotectants
Pulver, A. Shevtsov, B. Leybovich, I. Artyuhov, Y. Maleev,
and A. Peregudov

400-406

 

 


Coconut (Cocos Nucifera L.) Pollen cryopreservation
Anitha Karun, K.K. Sajini, V. Niral, C.H. Amarnath,
P. Remya, M.K. Rajesh, K. Samsudeen, B. A. Jerard
and Florent Engelmann

407-417

 

 


Low-temperature storage and cryopreservation of grapefruit (Citrus paradisi Macfad.) seeds
Nan Zhang, Bin Wen, Mingyue Ji and Qi Yan

418-426

 

 


Evaluation of cryoprotective effect ofturkish pine honey on common carp (Cyprinus carpio) spermatozoa
Fatih Ögretmen and Burak E. İnanan

427-437

 

 


Characterization and expression analysis of six chitinase Genes from the desert beetle Microdera punctipennis in Response to low temperature
Xueying Lu, Jieqiong Li, Xiaoning Liu, Xiaobo Li and Ji Ma

438-448

 

 


Letter to Editor: AMORPH 2014.
The Felix Franks Symposium: A Celebration
Harry Levine and Tony Auffret

449-450

 

 

 

 

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CryoLetters 35 (5), 356-360 (2014)
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EFFECT OF FASUDIL HYDROCHLORIDE ON THE POST-THAW VIABILITY OF CRYOPRESERVED PORCINE ADIPOSE-DERIVED STEM CELLS

Adipose-derived stem cells (ADSCs) are of interest for regenerative medicine as they are isolated easily and can differentiate into multiple cell lineages. Recently, it was reported that a Rho-associated kinase (ROCK) inhibitor Y-27632 could enhance the post-thaw viability and physiological function of cryopreserved BMSCOBJECTIVE: The present study  is to investigate whether Fasudil hydrochloride (FH,a selective Rho-kinase inhibitor like Y-27632) can exert a similar beneficial effect on the post-thaw viability of cryopreserved ADSCsMETHODS: ADSCs were allotted in three cryopreservation solutions [I:10% (v/v) (DMSO), 30% (v/v) FBS (as a negative control);II:10µM FH, 10% (v/v) DMSO, 30% (v/v) FBS;III:10µM Y-27632, 10% (v/v) DMSO, 30% (v/v) fetal bovine serum. All groups were frozen using a rapid freezing method and stored at -196° C in liquid nitrogen for 30 days.After thawing and being cultured 24h,viability of ADSCs were detected by MTT assay. RESULT: The MTT assay showed significant differences in the proportion of adherent viable cells over the concentration of Y-27632 and FH, Additionally, FH did not induce morphological changes in the frozen-thawed ADSCs like Y-27632 didCONCLUSIONS: FH might represent a promising cryopreservation solution in enhancing the post-thaw viability and physiological function of cryopreserved ADSCs and did not chang the cell appearance.

Fasudil hydrochloride, Y27632

 

 

 

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CryoLetters 35 (5), 361-370 (2014)
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alineferreirascarvalho@gmail.com

 

 

 

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CryoLetters 35 (5), 371-376 (2014)
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blliuk@163.com

 

 

 

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CryoLetters 35 (5), 377-384 (2014)
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cryohkim@sunchon.ac.kr

 

 

 

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CryoLetters 35 (5), 385-394 (2014)
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tuija.aronen@metla.fi

 

 

 

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CryoLetters 35 (5), 395-399 (2014)
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timothyhawes@hotmail.com

 

 

 

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CryoLetters 35 (5), 400-406 (2014)
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pulver.ibs@gmail.com

 

 

 

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CryoLetters 35 (5), 407-417 (2014)
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L.) pollen cryopreservation

kk.sajini@gmail.com) 

 

 

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CryoLetters 35 (5), 418-426 (2014)
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wenb@xtbg.org.cn

 

 

 

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CryoLetters 35 (5), 427-437 (2014)
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) spermatozoa

fatihogretmen@mu.edu.tr

 

 

 

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CryoLetters 35 (5), 438-448 (2014)
© CryoLetters, businessoffice@cryoletters.org

CHARACTERIZATION AND EXPRESSION ANALYSIS OF SIX CHITINASE GENES FROM THE DESERT BEETLE Microdera punctipennis IN RESPONSE TO LOW TEMPERATURE

Xueying Lu 1,2, Jieqiong Li 1, Xiaoning Liu 1, Xiaobo Li 2 and Ji Ma 1

1 Key Laboratory of Xinjiang Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqiô¬China.
2 Key Laboratory of Chemistry of Plant Resources in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, China.
*Corresponding author email: majiuci@xju.edu.cn

Abstract

BACKGROUND: Chitinase is responsible for chitin metabolism in a wide range of organisms. However, current knowledge on insect chitinase and their possible functions in relation to low temperature stress is very limited. OBJECTIVE: Six chitinase genes from cold treated desert beetle Microdera punctipennis obtained by RNA-seq technology were characterized, and their expression patterns in different tissues and in response to cold were investigated. METHODS: Multiple sequence alignment was carried out using ClustalW1.81 and Phylogenetic trees were generated by MEGA5. The expression patterns were studied by quantitative real-time PCR. RESULTS: These genes were belong to three different chitinase groups. Almost all of them were highly expressed in midgut, and some are expressed in fat body or hindgut. Subzero-4ºC had stronger effect than 4ºC in inducing chitinase expression. CONCLUSION: The tissue specific and cold inducible expressions suggest that the chitinases may have diverse functions and play roles in insect cold adaptation.

Keywords: Microdera punctipennis, chitinase genes, cold stress, tissue-specific gene expression.

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