CryoLetters

CryoLetters is a bimonthly, international journal for low temperature science and technology

	Volume 35, No. 5 September/October 2014 ISSN 0143-2044
	Effect of fasudil hydrochloride on the post-thaw viability of cryopreserved porcine adipose-derived stem cells Yuntao Ji, Deyu Chen, Shuanglin Jiang and Changqing Qu	356-360
	Efficacy of fish embryo vitrification protocols in terms of embryo morphology — a systematic review Aline Ferreira Souza de Carvalho, Shayenne Elizianne Ramos, Thatijanne Santos Gonzaga de Carvalho, Yasmin Chalfoun Pomárico de Souza, Márcio Gilberto Zangeronimo, Luciano José Pereira and Luis David Solis Murgas	361-370
	Effect of ultrasonic waves on the freezing rates of potatoes in degassed coolant and untreated coolant Deyang Yu and Baolin Liu	371-376
	Development of an encapsulation-vitrification protocol for Rubia akane (Nakai) hairy roots: a comparison with non-encapsulation Dong-Jin Shin, Hyo-Eun Lee, Chang-Hwan Bae, Sang-Un Park, Ho-Nam Kang and Haeng-Hoon Kim	377-384
	Cryopreservation of dormant in vivo-buds of hybrid aspen: timing as critical factor Tuija Aronen and Leena Ryynänen	385-394
	Extracellular ice phase transitions in insects Timothy C. Hawes	395-399
	Production of organ extracellular matrix using a freeze-thaw cycle employing extracellular cryoprotectants Pulver, A. Shevtsov, B. Leybovich, I. Artyuhov, Y. Maleev, and A. Peregudov	400-406
	Coconut (Cocos Nucifera L.) Pollen cryopreservation Anitha Karun, K.K. Sajini, V. Niral, C.H. Amarnath, P. Remya, M.K. Rajesh, K. Samsudeen, B. A. Jerard and Florent Engelmann	407-417
	Low-temperature storage and cryopreservation of grapefruit (Citrus paradisi Macfad.) seeds Nan Zhang, Bin Wen, Mingyue Ji and Qi Yan	418-426
	Evaluation of cryoprotective effect ofturkish pine honey on common carp (Cyprinus carpio) spermatozoa Fatih Ögretmen and Burak E. İnanan	427-437
	Characterization and expression analysis of six chitinase Genes from the desert beetle Microdera punctipennis in Response to low temperature Xueying Lu, Jieqiong Li, Xiaoning Liu, Xiaobo Li and Ji Ma	438-448
	Letter to Editor: AMORPH 2014. The Felix Franks Symposium: A Celebration Harry Levine and Tony Auffret	449-450

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CryoLetters 35 (5), 356-360 (2014)
© CryoLetters, businessoffice@cryoletters.org

EFFECT OF FASUDIL HYDROCHLORIDE ON THE POST-THAW VIABILITY OF CRYOPRESERVED PORCINE ADIPOSE-DERIVED STEM CELLS

Adipose-derived stem cells (ADSCs) are of interest for regenerative medicine as they are isolated easily and can differentiate into multiple cell lineages. Recently, it was reported that a Rho-associated kinase (ROCK) inhibitor Y-27632 could enhance the post-thaw viability and physiological function of cryopreserved BMSC.  OBJECTIVE: The present study  is to investigate whether Fasudil hydrochloride (FH,a selective Rho-kinase inhibitor like Y-27632) can exert a similar beneficial effect on the post-thaw viability of cryopreserved ADSCs.  METHODS: ADSCs were allotted in three cryopreservation solutions [I:10% (v/v) (DMSO), 30% (v/v) FBS (as a negative control);II:10µM FH, 10% (v/v) DMSO, 30% (v/v) FBS;III:10µM Y-27632, 10% (v/v) DMSO, 30% (v/v) fetal bovine serum. All groups were frozen using a rapid freezing method and stored at -196° C in liquid nitrogen for 30 days.After thawing and being cultured 24h,viability of ADSCs were detected by MTT assay. RESULT: The MTT assay showed significant differences in the proportion of adherent viable cells over the concentration of Y-27632 and FH, Additionally, FH did not induce morphological changes in the frozen-thawed ADSCs like Y-27632 did.  CONCLUSIONS: FH might represent a promising cryopreservation solution in enhancing the post-thaw viability and physiological function of cryopreserved ADSCs and did not chang the cell appearance.

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